CLINICAL-APPLICATIONS OF A DIRECT ASSAY OF FREE PROTEIN-S ANTIGEN USING MONOCLONAL-ANTIBODIES - A STUDY OF 59 CASES

被引:16
作者
WOLF, M
BOYERNEUMANN, C
PEYNAUDDEBAYLE, E
MARFAINGKOKA, A
AMIRAL, J
MEYER, D
机构
[1] HOP BICETRE,INSERM,U143,F-94270 LE KREMLIN BICETR,FRANCE
[2] SERBIO RES LAB,GENNEVILLIERS,FRANCE
关键词
FREE PROTEIN S; ELISA; MONOCLONAL ANTIBODIES; CONGENITAL PROTEIN S DEFICIENCY; ACQUIRED PROTEIN S DEFICIENCY;
D O I
10.1097/00001721-199404000-00005
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A new one-step ELISA using two monoclonal antibodies specific for distinct epitopes of the free form of protein S (ELISA-m) has been developed for the direct measurement of free protein S in untreated plasma. This assay has been compared with the classic method using polyclonal antibodies to protein S (ELISA-p). The latter method has the drawback of requiring PEG precipitation of plasma which is time-consuming, difficult to perform with accuracy and therefore poorly reproducible in most laboratories. Results of both ELISAs were compared with those of a functional assay. In 30 normal subjects, there was an excellent correlation between ELISA-m and ELISA-p (r = 0.95) as well as between ELISA-m and the functional assay (r = 0.96). In twelve patients with a congenital deficiency, the levels of free protein S antigen were similarly decreased with ELISA-m and ELISA-p and in good agreement with those of protein S activity. In 20 patients with miscellaneous inflammatory diseases, the levels of free proteins S were normal with good correlation between both ELISAs and PS activity, despite high levels of C4bBP-protein S complexes. As expected, in 15 dicoumarol-treated patients, there was a significant and parallel decrease of free protein S antigen with both ELISAs, with even lower levels of protein S activity. In 14 patients with liver cirrhosis, the mean values for free protein S antigen were normal using both assays, but with wide extreme values, whereas protein S activity was significantly lower. This new ELISA, is specific, reproducible, fast and easy to perform and provides a useful tool for the rapid diagnosis of congenital and acquired protein S deficiency.
引用
收藏
页码:187 / 192
页数:6
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