ASSESSMENT OF DISPARATE STRUCTURAL FEATURES IN 3 MODELS OF THE HEPATITIS-DELTA VIRUS RIBOZYME

被引：39

作者：

PERROTTA, AT ^{[1
]}

BEEN, MD ^{[1
]}

机构：

[1] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 17期

关键词：

D O I：

10.1093/nar/21.17.3959

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three models for the secondary structure of the hepatitis delta virus (HDV) antigenomic self-cleaving RNA element were tested by site-directed mutagenesis. Two models in which bases 5' to the cleavage site are paired with sequence at the 3' end of the element were both inconsistent with the data from the mutagenesis. Specifically, mutations in the 3' sequence which decrease self-cleavage activity could not be compensated by base changes in the 5' sequence as predicted by these models. The evidence was consistent with a third model in which the 3' end pairs with a portion of a loop within the ribozyme sequence to generate a pseudoknot structure. This same pairing was also required to generate higher rates of cleavage in trans with a 15-mer ribozyme, thus ruling out a proposed hammerhead-like 'axehead' model for the HDV ribozyme.

引用

页码：3959 / 3965

页数：7

共 24 条

[1] SECONDARY STRUCTURE OF THE SELF-CLEAVING RNA OF HEPATITIS DELTA-VIRUS - APPLICATIONS TO CATALYTIC RNA DESIGN [J].