EFFECTS OF ATRIAL-NATRIURETIC-PEPTIDE AND CGMP ON UPTAKE OF IGG COMPLEXES BY MACROPHAGES

被引:30
作者
MATTANA, J
SINGHAL, PC
机构
[1] LONG ISL JEWISH MED CTR,DEPT MED,DIV NEPHROL,RM 228,NEW HYDE PK,NY 11042
[2] YESHIVA UNIV ALBERT EINSTEIN COLL MED,BRONX,NY 10461
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 265卷 / 01期
关键词
PHAGOCYTOSIS; IMMUNOGLOBULIN-G COMPLEXES;
D O I
10.1152/ajpcell.1993.265.1.C92
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Macromolecular handling by macrophages and glomerular mesangial cells may be important in the development of renal injury. We undertook the present study to determine whether atrial natriuretic peptide (ANP), a particulate guanylate cyclase stimulator, plays a direct role in uptake of immunoglobulin G (IgG) complexes by macrophages. Macrophages incubated with ANP at 10(-5)and 10(-6) M showed significantly suppressed uptake of IgG complexes compared with control. Macrophage uptake of IgG complexes was also significantly suppressed by the soluble guanylate cyclase stimulator sodium nitroprusside. Dibutyryl guanosine 3',5'-cyclic monophosphate and dibutyryl adenosine 3',;5'-cyclic monophosphate both significantly suppressed IgG complex uptake as well. ANP was found to significantly enhance macrophage guanosine 3',5'-cyclic monophosphate (cGMP) levels compared with control cells, and this effect was antagonized by angiotensin II. Angiotensin II significantly enhanced uptake of IgG complexes and suppressed macrophage adenosine 3',5'-cyclic monophosphate synthesis, and both effects were antagonized by coincubation with ANP. These results suggest that ANP modulates uptake of IgG complexes by macrophages and that this effect may be mediated via cGMP.
引用
收藏
页码:C92 / C98
页数:7
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