EVALUATION OF DRUG LIPID ASSOCIATION CONSTANTS FROM MICROELECTROPHORETIC MOBILITY MEASUREMENTS

被引：9

作者：

BANERJEE, S

CASPERS, J

BENNOUNA, M

SAUTEREAU, AM

TOCANNE, JF

RUYSSCHAERT, JM

机构：

[1] UNIV ABDELMALEK ESSAADI,FAC SCI,DEPT BIOL,BIOCHIM & PHYSIOL LAB,TETOUAN,MOROCCO

[2] CNRS,DEPT GLYCOCONJUGUES & BIOMEMBRANES,PHARMACOL & TOXICOL FONDAMENTALES LAB,F-31062 TOULOUSE,FRANCE

[3] UNIV TOULOUSE 3,F-31062 TOULOUSE,FRANCE

来源：

LANGMUIR | 1995年 / 11卷 / 04期

关键词：

D O I：

10.1021/la00004a017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Electrophoretic mobilities of negatively charged lipid vesicles were measured after incubation with cationic drugs (adriamycin, celiptium, ethidium bromide, tetracaine) at different drug concentrations. We show here that comparison of the measured electrophoretic mobility with adsorption isotherms allows calculation of the drug-binding constants over a large range of drug concentrations, whatever the structure of the drug, its topology into the lipid bilayer, or its aggregation state.

引用

页码：1134 / 1137

页数：4

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