In order to reach a higher accuracy concerning the cytological locations of the rye seed storage protein loci Sec2[Gli-R2] and Sec3[Glu-R1] located within chromosome arms 2RS and 1RL, respectively, the linkage relationships between the following loci were analyzed: isozyme loci Gpi-R1, Mdh-R1, and Pgd2, translocation T273W (Wageningen tester set, involving chromosome arms 1RS and 5RL), the telomere C-bands of chromosome arms 1RL (tL1), 2RS (tS2), and 5RS (tS5), and three interstitial C-bands in chromosome arm 1RS (iS1), in the middle of chromosome arm 1RL(iL1), and in the middle of chromosome arm 2RL (iL2), respectively. The data indicated that locus Sec3 is located in the distal half of chromosome arm 1RL (between C-band iL1 and locus Pgd2), while locus Sec2 is located a short distance (2.9 +/- 1.4%) from the telomere C-band of chromosome arm 2RS.