Oligonucleotide probes based on 16S rRNA sequences for the identification of four Azospirillum species

被引:17
作者
Kabir, MM
Faure, D
Haurat, J
Normand, P
Jacoud, C
Wadoux, P
Bally, R
机构
[1] UNIV LYON 1,LAB ECOL MICROBIENNE SOL,URA CNRS 1450,F-69622 VILLEURBANNE,FRANCE
[2] LYONNAISE IND PHARMACEUT,F-69330 MEYZIEU,FRANCE
关键词
Azospirillum; bacterial isolation; hybridization; oligonucleotide probe; sequence analysis; 16S rRNA;
D O I
10.1139/m95-151
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Partial sequences of the 16S rRNA molecules of nine strains belonging to four Azospirillum species were used to design species-specific oligonucleotide probes. Azospirillum strains sequences were analyzed and three homologous fragments containing 16 nucleotides were determined. These three probes were found to be characteristic of A. lipoferum (Al), A. irakense (Ai), and A, brasilense/amazonense species (Aba) and of few nontarget organisms. The specificity of these three probes was tested both against sequences in the GenBank data base and in numerous colony hybridization experiments. As a few nontarget organisms hyridized with the different Azospirillum probes, the use of these probes in bulk soil hybridization is not permitted. However, their use together with specific isolation techniques is validated.
引用
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页码:1081 / 1087
页数:7
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