LARGE SCALE PREPARATION OF A CRUDE MEMBRANE FRACTION FROM OX BRAIN

Abstract— Two procedures are described for preparing from ox brain grey matter a subcellular fraction enriched in neuronal membranes. In the first, an angle rotor was used to fractionate the crude mitochondrial and nuclear fraction and yielded 1·3–1·4 g of membrane protein from 200 g of tissue. The second procedure employed essentially the same principle except that a zonal rotor was used to fractionate in the final stage. The two methods gave comparable results for yields of protein, Na‐K‐Mg AT Pase and succinate dehydrogenase. Copyright © 1969, Wiley Blackwell. All rights reserved