EXPRESSION OF DNA LIGASE-I AND LIGASE-II DURING OOGENESIS AND EARLY DEVELOPMENT OF XENOPUS-LAEVIS

被引：8

作者：

AOUFOUCHI, S ^{[1
]}

PRIGENT, C ^{[1
]}

THEZE, N ^{[1
]}

PHILIPPE, M ^{[1
]}

THIEBAUD, P ^{[1
]}

机构：

[1] UNIV RENNES 1,BIOL & GENET DEV LAB,CNRS,URA 256,CAMPUS BEAULIEU,F-35042 RENNES,FRANCE

来源：

DEVELOPMENTAL BIOLOGY | 1992年 / 152卷 / 01期

关键词：

D O I：

10.1016/0012-1606(92)90171-C

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have analyzed the expression of DNA ligase I protein during oogenesis and early development of Xenopus laevis. The protein is already present in stage I oocytes and then accumulates throughout oogenesis to reach a steady state level by stage VI. It remains at this level at least until tadpole stage. In stage VI oocytes DNA ligase I protein is almost exclusively localized in the germinal vesicle. We have partially purified a DNA ligase II activity from stage VI oocytes, unfertilized eggs, and stage 8 embryos. An 80-kDa polypeptide can be specifically adenylated in all three purified extracts. It is not recognized by antibodies directed against DNA ligase I and is active on oligo(dT)-poly(rA) substrate. It could therefore represent DNA ligase II protein. The presence of both DNA ligases I and II in oocytes and embryos is inconsistent with the DNA ligase model that had been previously proposed for amphibia. © 1992.

引用

页码：199 / 202

页数：4