ENHANCED READTHROUGH OF OPAL (UGA) STOP CODONS AND PRODUCTION OF MYCOPLASMA-PNEUMONIAE P1 EPITOPES IN ESCHERICHIA-COLI

被引：34

作者：

SMILEY, BK ^{[1
]}

MINION, FC ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,VET MED RES INST,DEPT MICROBIOL IMMUNOL & PREVENT MED,AMES,IA 50011

来源：

GENE | 1993年 / 134卷 / 01期

关键词：

CODON USAGE; SUPPRESSOR TRANSFER RNA; TRPT76; UROGENITAL TRACT INFECTIONS;

D O I：

10.1016/0378-1119(93)90171-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Expression of mycoplasma sequences in Escherichia coli is often hindered by an unusual mycoplasmal codon usage pattern: the UGA stop codon is utilized for tryptophan. This may result in the truncation of cloned proteins and may prevent the detection of products of many cloned genes. To circumvent this translation barrier, we have developed an expression system for the production of mycoplasma proteins in E. coli. The efficiency of an opal suppressor tRNA (trp T176) was augmented with other suppressor mutations {prfB3 or rrsB(SuUGA-DELTAC1054)} which influence termination events. System efficacy was analyzed by employing suppressor mutations in the expression of TGA-containing sequences from the PI protein-encoding gene of Mycoplasma pneumoniae.

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页码：33 / 40

页数：8

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