COMPARATIVE-ANALYSIS OF 2 ASSAYS FOR GENOTYPING HEPATITIS-C VIRUS-BASED ON GENOTYPE-SPECIFIC PRIMERS OR PROBES

被引：59

作者：

GIANNINI, C

THIERS, V

NOUSBAUM, JB

STUYVER, L

MAERTENS, G

BRECHOT, C

机构：

[1] CHU NECKER ENFANTS MALAD,U370,F-75730 PARIS 15,FRANCE

[2] INST PASTEUR,CBMS,PARIS,FRANCE

[3] INNOGENET NV,GHENT,BELGIUM

[4] UNIV FLORENCE,IST MED INTERNA,FLORENCE,ITALY

来源：

JOURNAL OF HEPATOLOGY | 1995年 / 23卷 / 03期

关键词：

DIRECT SEQUENCING; HEPATITIS C VIRUS GENOTYPES; HCV-RNA; LIPA; POLYMERASE CHAIN REACTION;

D O I：

10.1016/S0168-8278(95)80002-6

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims: The importance of determining the hepatitis C virus genotype has increased since several authors have described a good correlation between hepatitis C virus genotype and response to interferon treatment or to disease severity, It is thus of particular importance to develop reliable assays for hepatitis C virus genotyping. Methods: We have comparatively analyzed hepatitis C virus genotypes of 208 French and Italian chronically infected patients using genotype-specific primers polymerase chain reaction in the capsid region and a genotype-specific probe-based assay in the 5' untranslated region (LiPA). Results: We found a good concordance between the two assays for the prevalent genotypes in our regions (145/174). The nucleotide sequences in the 5'UTR and capsid domain mere investigated to determine the molecular basis of some discordant results, This analysis showed that the genotype-specific probe-based assay gave more consistent results, probably because this technique is based on several probes distributed along the 5'UTR to reveal each genotype, In contrast, the low variability of 5'UTR did not always permit classification of the hepatitis C virus genotype 1 subtypes, We also found some problems, using genotype-specific primers polymerase chain reaction, for less represented genotypes; in particular, in the presence of type 2a/III we obtained more discordant results, This observation is of importance in view of the potential association of this genotype with mild liver disease and a good response to interferon-a treatment.

引用

页码：246 / 253

页数：8

共 30 条

[1] Choo, Kuo, Weiner, Overby, Bradley, Houghton, Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome, Science, 244, pp. 359-362, (1989)
[2] Choo, Richman, Han, Berger, Lee, Dong, Gallegos, Coit, Medina-Selby, Weiner, Bradley, Kuo, Houghton, Genetic organization and diversity of the hepatitis C virus, Proc Natl Acad Sci USA, 88, pp. 2451-2455, (1991)
[3] Cha, Beall, Irvine, Kolberg, Kuo, Urdea, At least five related, but distinct, hepatitis C viral genotypes exist, Proc Natl Acad Sci USA, 89, pp. 7144-7148, (1992)
[4] Chan, McOmish, Holmes, Dow, Peutherer, Follett, Yap, Simmonds, Analysis of a new HCV type and its phylogenetic relationship to existing variants, Journal of General Virology, 273, pp. 1131-1141, (1992)
[5] Enomoto, Takada, Nakao, Date, There are two major types of HCV in Japan, Biochem Biophys Res Commun, 170, pp. 1021-1025, (1990)
[6] McOmish, Chan, Dow, Gillon, Frame, Crawford, Yap, Follett, Simmonds, Detection of three types of hepatitis C virus in blood donors: investigation of typespecific differences in serologic reactivity and rate of alanine aminotransferase abnormalities, Transfusion, 33, pp. 7-13, (1993)
[7] Okamoto, Sugiyama, Okata, Kurai, Akahane, Sugai, Tanaka, Sato, Tsuda, Miyakawa, Mayumi, Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources, J Gen Virol, 73, pp. 673-679, (1992)
[8] Okamoto, Tokita, Sakamoto, Horikita, Kojima, Iisuka, Mishiro, Characterization of the genomic sequence of type-V (or 3a) hepatitis C virus isolates and PCR primers for specific detection, J Gen Virol, 74, pp. 2385-2390, (1993)
[9] Simmonds, McOmish, Yap, Chan, Lin, Dusheiko, Saeed, Holmes, Sequence variability in the 5′ noncoding region of hepatitis C virus: identification of a new virus type and restrictions on sequence diversity, J Gen Virol, 74, pp. 661-668, (1993)
[10] Simmonds, Holmes, Cha, Cham, McOmish, Irvine, Beall, Yap, Kolberg, Urdea, Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS5 region, J Gen Virol, 74, pp. 2391-2399, (1993)

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