OPTIMIZED PLANT-REGENERATION FROM CALLUS DERIVED FROM SEEDLING HYPOCOTYLS OF EUCALYPTUS-UROPHYLLA

被引：35

作者：

TIBOK, A ^{[1
]}

BLACKHALL, NW ^{[1
]}

POWER, JB ^{[1
]}

DAVEY, MR ^{[1
]}

机构：

[1] UNIV NOTTINGHAM, DEPT LIFE SCI, PLANT GENET MANIPULAT GRP, NOTTINGHAM NG7 2RD, ENGLAND

来源：

PLANT SCIENCE | 1995年 / 110卷 / 01期

关键词：

EUCALYPTUS UROPHYLLA; HYPOCOTYL EXPLANTS; SHOOT REGENERATION; PLOIDY;

D O I：

10.1016/0168-9452(95)04188-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Shoots were regenerated from callus initiated from hypocotyl explants of 14-day-old seedlings of Eucalyptus urophylla. Organogenesis was the pathway of shoot regeneration on Murashige and Skoog (MS)-based medium supplemented with cr-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP); NAA in combination with thidiazuron (N-phenyl-N'-1,2,3-thidiazol-5-yl urea; TDZ) induced both organogenesis and the production of heart-shaped embryo-like structures. Explants from seeds of different geographical origins exhibited differences in shoot regeneration frequencies. Somaclonal variation was observed amongst regenerated plants. Additionally, 28% of the explant-derived plants were haploid, while 12% were triploid.

引用

页码：139 / 145

页数：7

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