EVALUATION OF MEDIATORS FOR DEVELOPMENT OF AMPEROMETRIC MICROBIAL BIOELECTRODES

被引：25

作者：

KALAB, T ^{[1
]}

SKLADAL, P ^{[1
]}

机构：

[1] MASARYK UNIV,DEPT BIOCHEM,CR-61137 BRNO,CZECH REPUBLIC

来源：

ELECTROANALYSIS | 1994年 / 6卷 / 11-12期

关键词：

BIOSENSOR; MICROBIAL ELECTRODE; CARBON PASTE; ELECTROCATALYSIS; FERROCENE; LACTATE; SUCCINATE;

D O I：

10.1002/elan.1140061114

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The microbial biosensors were constructed from carbon paste electrodes (CPEs) modified with insoluble mediators and immobilized membrane vesicles or whole cells of the bacterium Paracoccus denitrificans. The best results were obtained with membrane vesicles and ferrocene (FC). This system could serve for determination of succinate, D-lactate, and L-lactate. Significant increase of signal was obtained when competitive flux of electrons to oxygen was blocked by inhibiting terminal oxidases using cyanide.

引用

页码：1004 / 1008

页数：5

共 15 条

[1]

ADAMOWICZ E, 1988, BIOSENSORS, V3, P27

[2]

BENNETTO HP, 1987, BIOSENSORS FUNDAMENT, P291

[3] MEDIATOR COMPOUNDS FOR THE ELECTROCHEMICAL STUDY OF BIOLOGICAL REDOX SYSTEMS - A COMPILATION [J].