GENE GUN-BASED NUCLEIC-ACID IMMUNIZATION - ELICITATION OF HUMORAL AND CYTOTOXIC T-LYMPHOCYTE RESPONSES FOLLOWING EPIDERMAL DELIVERY OF NANOGRAM QUANTITIES OF DNA

被引：244

作者：

PERTMER, TM ^{[1
]}

EISENBRAUN, MD ^{[1
]}

MCCABE, D ^{[1
]}

PRAYAGA, SK ^{[1
]}

FULLER, DF ^{[1
]}

HAYNES, JR ^{[1
]}

机构：

[1] AGRACETUS INC,MIDDLETON,WI 53562

来源：

VACCINE | 1995年 / 13卷 / 15期

关键词：

GENE GUN; DNA IMMUNIZATION; INFLUENZA; SKIN;

D O I：

10.1016/0264-410X(95)00069-D

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Particle-mediated (gene gun) DNA transfer to the epidermis was evaluated for its ability to elicit humoral and cytotoxic T lymphocyte responses using decreasing quantities of plasmid DNA-based antigen expression vectors. Using plasmids encoding human growth hormone, human alpha-1-antitrypsin, and influenza virus nucleoprotein, strong immune responses were observed in mice following immunization with as little as 16 ng of DNA using an electric discharge gene delivery system. Significant antibody titers were observed against these antigens following a primary immunization, with responses rising dramatically following a boost. Increasing the DNA dose above 16 ng per immunization had little beneficial effect. In contrast to particle-mediate DNA delivery, intramuscular or intradermal inoculation required greater than 5000-fold more DNA to achieve comparable results. Data are also presented demonstrating that a simple, hand-held version of the Accell(R) DNA delivery system, employing compressed helium as the particle motive force, achieves immune responses comparable to the traditional electric discharge device.

引用

页码：1427 / 1430

页数：4

共 9 条

[1] DNA-BASED IMMUNIZATION INDUCES CONTINUOUS SECRETION OF HEPATITIS-B SURFACE-ANTIGEN AND HIGH-LEVELS OF CIRCULATING ANTIBODY [J].