BIOSYNTHESIS OF 3-NITROPROPIONIC ACID IN CREEPING INDIGO (INDIGOFERA SPICATA)

被引：21

作者：

CANDLISH, E

LACROIX, LJ

UNRAU, AM

机构：

[1] Department of Plant Science, University of Manitoba

[2] Department of Chemistry, Simon Fraser University

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 01期

关键词：

D O I：

10.1021/bi00829a026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A study of the biosynthesis of 3-nitropropionic acid in Indigofera spicata (Jacq.) Forsk. was undertaken by feeding14C-labeled precursors to intact plants or to cuttings. An organic acid fraction containing 3-nitropropionic acid was obtained by extraction of the plant material and separation by column chromatography. The 3-nitropropionic acid and other major organic acids were separated and analyzed quantitatively by gas-liquid partition chromatography. No evidence was obtained to suggest that Krebs cycle acids were precursors of 3-nitropropionic acid. Malonic acid or a derivative of malonate appears to be a fairly immediate precursor since14C was found in 3-nitropropionic acid when malonate-2-14C or malonyl itionohydroxamate- 2-14C were fed as precursors. © 1969, American Chemical Society. All rights reserved.

引用

页码：182 / &

共 31 条

[1] BIRCH AJ, 1960, CHEM IND-LONDON, P840
[2] STUDIES IN BIOCHEMISTRY OF MICRO-ORGANISMS .116. BIOSYNTHESIS OF BETA-NITROPROPIONIC ACID BY MOULD PENICILLIUM ATROVENETUM G SMITH
BIRKINSHAW, JH
DRYLAND, AML
[J]. BIOCHEMICAL JOURNAL, 1964, 93 (03) : 478 - +
[3] SYNTHESIS OF ALPHA-KETOGLUTARATE FROM SUCCINATE AND CARBON DIOXIDE BY A SUBCELLULAR PREPARATION OF A PHOTOSYNTHETIC BACTERIUM
BUCHANAN, BB
EVANS, MCW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 54 (04) : 1212 - &
[4] BUSH MT, 1951, J BIOL CHEM, V118, P685
[5] ANALYSIS OF SOME ORGANIC ACIDS BY GAS-LIQUID CHROMATOGRAPHY
CANVIN, DT
[J]. CANADIAN JOURNAL OF BIOCHEMISTRY, 1965, 43 (08): : 1281 - &
[6] HIPTAGENIC ACID IDENTIFIED AS BETA-NITROPROPIONIC ACID
CARTER, CL
MCCHESNEY, WJ
[J]. NATURE, 1949, 164 (4170) : 575 - 576
[7] CARTER CL, 1943, J SOC CHEM IND LOND, V62, pT238
[8] COLOWICK SP, 1962, METHODS ENZYMOL, V4, P718
[9] COLOWICK SP, 1962, METHODS ENZYMOL, V5, P444
[10] DE VELLIS J., 1963, PLANT PHYSIOL, V38, P686, DOI 10.1104/pp.38.6.686

← 1 2 3 4 →