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FLUOROMETRIC SUBSTRATE FOR SULFATASE AND LIPASE

被引：59
作者：
GUILBAULT, GG
HIESERMAN, J
机构：
[1] Department of Chemistry, Louisiana State University, Lakefront Campus, New Orleans
来源：
ANALYTICAL CHEMISTRY | 1969年 / 41卷 / 14期
关键词：
D O I：
10.1021/ac50159a034
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
New fluorometric substrates are described for the assay of the enzyme sulfatase and lipase. β-Naphthol sulfate and 4-methyl umbelllferone sulfate are useful for the assay of sulfatases and N-methyl indoxyl myristate is an excellent substrate for the analysis of lipase. As little as 10-4 unit per ml of lipase or 10-3 unit per ml of sulfatase can be determined by a direct reaction rate method in 2-3 minutes with a precision and accuracy of about 1.5%. © 1969, American Chemical Society. All rights reserved.
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页码：2006 / +
页数：1
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