EPIDERMAL GROWTH-FACTOR AND ITS RECEPTORS IN HUMAN PANCREATIC-CARCINOMA

被引：34

作者：

CHEN, YF

PAN, GZ

HOU, X

LIU, TH

CHEN, J

YANAIHARA, C

YANAIHARA, N

机构：

[1] Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing

[2] Osaka University Hospital, Osaka

[3] University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka

来源：

PANCREAS | 1990年 / 5卷 / 03期

关键词：

Epidermal growth factor; Epidermal growth factor receptors; Pancreatic carcinoma; Radioimmunoassay; Radioreceptor assay;

D O I：

10.1097/00006676-199005000-00006

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The role of epidermal growth factor (EGF) in oncogenesis and progression of malignant tumors is a subject of vast interest. In this study, radio-immunoassay and radioreceptor assay of EGF were established. EGF contents in malignant and benign pancreatic tumors, in normal pancreas tissue, and in culture media of a human pancreatic carcinoma cell line were determined. EGF receptor binding studies were performed. It was shown that EGF contents in pancreatic carcinomas were significantly higher than those in normal pancreas or benign pancretic tumors. EGF was also detected in the culture medium of a pancreatic carcinoma cell line. The binding of125I-EGF to the pancreatic carcinoma cells was time and temperature dependent, reversible, competitive, and specific. Scatchard analysis showed that the dissociation constant of EGF receptor was 2.1 x 10-9Af, number of binding sites was 1.3 x 105cell. These results indicate that there is an over-expression of EGF/EGF receptors in pancreatic carcinomas, and that an autocrine regulatory mechanism may exist in the growth-promoting effect of EGF on tumor cells. © 1990 Raven Press, New York.

引用

页码：278 / 283

页数：6

共 18 条

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