SENSITIVE BIOSENSOR FOR CHOLINE AND ACETYLCHOLINE INVOLVING FAST IMMOBILIZATION OF A BIENZYME SYSTEM ON A DISPOSABLE MEMBRANE

被引：65

作者：

MORELIS, RM ^{[1
]}

COULET, PR ^{[1
]}

机构：

[1] UNIV LYON 1,LAB GENIE ENZYMAT,ATELIER BIOTECHNOL,CNRS,UMR 106,F-69622 VILLEURBANNE,FRANCE

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 231卷 / 01期

关键词：

D O I：

10.1016/S0003-2670(00)86393-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biosensor was prepared for the determination of choline or acetylcholine by co-immobilizing choline oxidase and cholinesterase on a chemically preactivated membrane ready for use. This rapid procedure allows the coupling to be performed in a few minutes. The determination is based on the electrochemical detection of enzymatically generated hydrogen peroxide. This sensor has a detection limit of 5 × 10-8 M. The response was obtained in 2 min and was linear up to 2 × 10-5 M. © 1990.

引用

页码：27 / 32

页数：6

共 24 条

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