RECOMMENDED PROTOCOLS BASED ON A SURVEY OF CURRENT PRACTICE IN GENOTOXICITY TESTING LABORATORIES .3. CELL-TRANSFORMATION IN C3H/10T1/2 MOUSE EMBRYO CELL, BALB/C 3T3 MOUSE FIBROBLAST AND SYRIAN-HAMSTER EMBRYO CELL-CULTURES

被引：35

作者：

DUNKEL, VC

ROGERS, C

SWIERENGA, SHH

BRILLINGER, RL

GILMAN, JPW

NESTMANN, ER

机构：

[1] HLTH & WELF CANADA,HLTH PROTECT BRANCH,BUR DRUG RES,TUNNEYS PASTURE,OTTAWA K1A 0L2,ONTARIO,CANADA

[2] UNIV GUELPH,GUELPH N1G 2W1,ONTARIO,CANADA

[3] CANTOX INC,OAKVILLE L6J 5Z7,ONTARIO,CANADA

[4] HLTH & WELF CANADA,HLTH PROTECT BRANCH,FOOD DIRECTORATE,OTTAWA K1A 0L2,ONTARIO,CANADA

[5] US FDA,CTR FOOD SAFETY & APPL NUTR,WASHINGTON,DC 20204

来源：

MUTATION RESEARCH | 1991年 / 246卷 / 02期

关键词：

RECOMMENDED PROTOCOL; CURRENT USAGE; CELL TRANSFORMATION; C3H10/T1/2; BALB/C; 3T3; SHE CELLS;

D O I：

10.1016/0027-5107(91)90049-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A standardized protocol and guidelines for the performance of cell transformation testing in mouse embryo (C3H/10T1/2), mouse fibroblast (BALB/c 3T3) and Syrian hamster embryo (SHE) cells have been developed. The protocol is based primarily on current laboratory practices as determined by responses to a detailed questionnaire completed by North American and European governmental, university and contract laboratories involved with cell transformation experimentation. This report identifies those modifications to previously described methodologies which are being used on a regular basis and also serves to clarify confusing or inconsistent practices.

引用

页码：285 / 300

页数：16

共 29 条

[1]

BARRETT, 1986, CITED INDIRECTLY

[2]

BARRETT JC, 1986, IARC SCI PUBL, V83, P267

[3]

BARRETT JC, 1985, EVALUATION SHORT TER, P623

[4]

BARRETT JC, 1985, AGE RELATED FACTORS, P181