NORMAL SURVIVAL AND INVITRO DEVELOPMENT AFTER CRYOPRESERVATION OF ZONA-DRILLED EMBRYOS IN MICE

被引：12

作者：

DEPYPERE, HT

CARROLL, JC

VANDEKERCKHOVE, D

MATTHEWS, CD

机构：

[1] Department of Gynaecology and Obstetrics, University Hospital, 9000 Ghent

[2] Reproductive Medicine Unit, Queen Elizabeth Hospital, Woodville 5011, South

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 03期

关键词：

CRYOPRESERVATION; EMBRYOS; ZONA DRILLING;

D O I：

10.1093/oxfordjournals.humrep.a137353

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The importance of the zona pellucida on survival after freezing and thawing was investigated. Zona-drilled and zona-intact mouse embryos were fertilized in vitro, cultured to the 2, 4 and 8-cell stages and frozen using conventional methods. Zona drilling did not affect the survival or development of frozen embryos to the blastocyst stage in vitro. We conclude that partial damage to the zona pellucida during micro-manipulation procedures is compatible with rates of survival and development which are not different to those observed in zona-intact control embryos.

引用

页码：432 / 435

页数：4

共 22 条

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