SURFACE-PLASMON STUDIES OF SPECIFIC RECOGNITION REACTIONS AT SELF-ASSEMBLED MONOLAYERS ON GOLD

被引：39

作者：

SCHMITT, FJ ^{[1
]}

HAUSSLING, L ^{[1
]}

RINGSDORF, H ^{[1
]}

KNOLL, W ^{[1
]}

机构：

[1] UNIV MAINZ,INST ORGAN CHEM,W-6500 MAINZ,GERMANY

来源：

THIN SOLID FILMS | 1992年 / 210卷 / 1-2期

关键词：

D O I：

10.1016/0040-6090(92)90412-5

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Specific recognition reactions of proteins at functionalized interfaces were observed with plasmon surface polariton (PSP) spectroscopy. This optical method offers a vertical resolution of about 0.2 nm in aqueous media. Different types of receptor surfaces were prepared, either by self-assembly of biotinylated alkane thiols or by specific adsorption of streptavidin monolayers. The corresponding substrates were streptavidin and a biotinylated monoclonal antibody. Their binding properties are inversely related to the packing density of the receptors. A good accessibility is only given in the case of a "diluted" presentation of the receptors at the interface. Both binding site and diffusion limited adsorption have been observed.

引用

页码：815 / 817

页数：3

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