RETENTION OF SOME RING-SUBSTITUTED ANILINE DERIVATIVES BY POROUS GRAPHITIZED CARBON - DEPENDENCE ON PHYSICOCHEMICAL PARAMETERS

The retention characteristics of 22 aniline derivatives were determined on a porous, graphitized-carbon column in unbuffered acetonitrile-water and methanol-water mixtures. Each aniline derivative gave symmetrical peaks in each eluent without buffers. Good linear correlations were found between the log k' and the concentration of the organic component in the eluent. The slope and intercept values differed according to the type of organic modifier and the character, number and position of substituents, indicating the different selectivities of methanol and acetonitrile and the good separating power of the column. Multivariate mathematical-statistical calculations proved that the retention of ring-substituted aniline derivatives is mainly governed by electronic parameters and the hydrogen acceptor capacity of substituents.