THE ROLE OF A PHOSPHATIDYLCHOLINE-SPECIFIC PHOSPHOLIPASE-C IN THE PRODUCTION OF DIACYLGLYCEROL FOR NITRIC-OXIDE SYNTHESIS IN MACROPHAGES ACTIVATED BY IFN-GAMMA AND LPS

被引：46

作者：

SANDS, WA ^{[1
]}

CLARK, JS ^{[1
]}

LIEW, FY ^{[1
]}

机构：

[1] UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G11 6NT,SCOTLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 199卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1251

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Murine macrophages activated by interferon (IFN)-gamma and bacterial lipopolysaccharide (LPS) produce large amounts of nitric oxide (NO), which is a critical mediator for a variety of biological functions. The expression of this inducible NO synthase (iNOS) involves a protein kinase C (PKC)-dependent pathway, but the mechanism for the PKC activation in this system is unclear. Through analysis of diacylglycerol (DAG) synthesis and choline metabolism in activated macrophages, direct evidence is provided that NO synthesis involves the activation of an unusual phosphatidylcholine-specific phospholipase C (PC-PLC) and not a phosphatidylinositol-specific phospholipase C (PI-PLC) or phospholipase D (PLD). (C) 1994 Academic Press, Inc.

引用

页码：461 / 466

页数：6

共 19 条

[1] EFFECTS OF CHOLINERGIC STIMULATION ON LEVELS AND FATTY-ACID COMPOSITION OF DIACYLGLYCEROLS IN MOUSE PANCREAS [J].