NEURITE-PROMOTING ACTIVITY OF COLLAGENS ON EMBRYONIC NEURONS - DECREASED EFFECT AT THE POSTNATAL STAGE

We studied the in vitro neurite outgrowth activity of fibronectin, laminin, heparan sulfate proteoglycan, type I collagen, type IV collagen, and type VIII collagen in cholinergic neuronal cell lines and primary cultured neurons. All these substances had high neurite promoting activity on primary cultured neurons from embryonic mouse brain. However, collagens had no such an effect on primary cultured neurons from postnatal brain. When neuronal cell lines were used, collagens and other extracellular matrix substances were equally and highly effective on cells originated from embryonic brain, but collagens were less effective on cells from postnatal brain. These findings suggest that postnatal neurons lose the neuritic responsiveness to collagens earlier than that of other ECM.