HIGH-PERFORMANCE HYDROPHOBIC INTERACTION CHROMATOGRAPHY OF PROTEINS ON TSKGEL PHENYL-5PW PREPARATIVE COLUMN

被引：19

作者：

KATO, Y

KITAMURA, T

HASHIMOTO, T

机构：

[1] Toyo Soda Manufacturing Co, Yamaguchi, Jpn, Toyo Soda Manufacturing Co, Yamaguchi, Jpn

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1986年 / 9卷 / 15期

关键词：

CHEMICAL ANALYSIS - Equipment - CHROMATOGRAPHIC ANALYSIS - Column - ENZYMES - Processing;

D O I：

10.1080/01483918608074179

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

TSKgel Phenyl-5PW preparative column of 200 multiplied by 55 mm I. D. was evaluated with respect to resolution, sample loading capacity and applications to the purification of enzymes. The preparative column provided similar separations as analytical column (75 multiplied by 7. 5 mm I. D. ) and 150 multiplied by 21. 5 mm I. D. preparative column. The sample loading capacity was 200 - 1000 mg depending on the sample. If the slight decrease in resolution is acceptable, much more samples could be applied. Lipoxidase, phosphoglucose isomerase and lactate dehydrogenase could be purified to a great extent with high recovery of activity (more than 80 %).

引用

页码：3209 / 3224

页数：16

共 16 条

[1]

Aebi H.E., 1983, METHOD ENZYMAT AN, VIII, P273, DOI DOI 10.1016/B978-0-12-091302-2.50032-3

[2] SILICA-BOUND POLYETHYLENEGLYCOL AS STATIONARY PHASE FOR SEPARATION OF PROTEINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].