LIGAND DYNAMICS IN THE PHOTODISSOCIATION OF CARBOXYHEMOGLOBIN BY SUBPICOSECOND TRANSIENT INFRARED-SPECTROSCOPY

被引：15

作者：

ROTHBERG, L ^{[1
]}

JEDJU, TM ^{[1
]}

AUSTIN, RH ^{[1
]}

机构：

[1] PRINCETON UNIV,PRINCETON,NJ 08544

来源：

BIOPHYSICAL JOURNAL | 1990年 / 57卷 / 02期

关键词：

D O I：

10.1016/S0006-3495(90)82538-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Time-resolved infrared spectroscopy with 0.5-ps resolution is used to track the evolution of the CO stretching vibration after visible photoexcitation of carboxyhemoglobin in water at room temperature. Polarization measurements determine that the iron-complexed CO is oriented nearly perpendicular to the porphyrin plane. The dissociation appears to proceed via a metastable excited state with 2 +/- 1 ps lifetime. The dissociated CO binds weakly in the heme pocket for at least 500 ps. This state correlates with the internally bound state observed by Frauenfelder et al. at low temperatures in myoglobin. © 1990, The Biophysical Society. All rights reserved.

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页码：369 / 373

页数：5

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