CRYSTALLIZATION OF THE GLOBULAR DOMAIN OF HISTONE-H5

被引：17

作者：

GRAZIANO, V ^{[1
]}

GERCHMAN, SE ^{[1
]}

WONACOTT, AJ ^{[1
]}

SWEET, RM ^{[1
]}

WELLS, JRE ^{[1
]}

WHITE, SW ^{[1
]}

RAMAKRISHNAN, V ^{[1
]}

机构：

[1] UNIV ADELAIDE,DEPT BIOCHEM,ADELAIDE,SA 5001,AUSTRALIA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 212卷 / 02期

关键词：

D O I：

10.1016/0022-2836(90)90122-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The globular domain of histone H1 H5 binds to the nucleosome and is crucial for the formation of chromatin higher order structure. We have expressed in Escherichia coli a gene that codes for the globular domain of H5. The protein produced in E. coli is functional in nucleosome binding assays. We have obtained crystals of the protein that diffract to beyond 2.5 Å (1 Å = 0.1 nm) resolution. The crystals are orthorhombic with unit cell dimensions of a = 80.1 a ̊A, b = 67.5 A ̊ and c = 38.0 A ̊. © 1990.

引用

页码：253 / 257

页数：5

共 17 条

[1] THE STRUCTURE OF HISTONE-H1 AND ITS LOCATION IN CHROMATIN [J].

ALLAN, J ;

HARTMAN, PG ;

CRANEROBINSON, C ;

AVILES, FX .

NATURE, 1980, 288 (5792) :675-679

[2] CONFORMATION OF HISTONE-H5 - ISOLATION AND CHARACTERIZATION OF GLOBULAR SEGMENT [J].

AVILES, FJ ;

CHAPMAN, GE ;

KNEALE, GG ;

CRANEROBINSON, C ;

BRADBURY, EM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 88 (02) :363-371

[3] CHICKEN ERYTHROCYTE HISTONE-H5 .4. SEQUENCE OF THE CARBOXY-TERMINED HALF OF THE MOLECULE (96 RESIDUES) AND COMPLETE SEQUENCE [J].

BRIAND, G ;

KMIECIK, D ;

SAUTIERE, P ;

WOUTERS, D ;

BORIELOY, O ;

BISERTE, G ;

MAZEN, A ;

CHAMPAGNE, M .

FEBS LETTERS, 1980, 112 (02) :147-151

[4] THE POLYPEPTIDE FOLD OF THE GLOBULAR DOMAIN OF HISTONE-H5 IN SOLUTION - A STUDY USING NUCLEAR-MAGNETIC-RESONANCE, DISTANCE GEOMETRY AND RESTRAINED MOLECULAR-DYNAMICS [J].

CLORE, GM ;

GRONENBORN, AM ;

NILGES, M ;

SUKUMARAN, DK ;

ZARBOCK, J .

EMBO JOURNAL, 1987, 6 (06) :1833-1842

[5] RECONSTITUTION OF CHROMATIN HIGHER-ORDER STRUCTURE FROM HISTONE-H5 AND DEPLETED CHROMATIN [J].

GRAZIANO, V ;

GERCHMAN, SE ;

RAMAKRISHNAN, V .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (04) :997-1007

[6] STUDIES ON ROLE AND MODE OF OPERATION OF VERY LYSINE-RICH HISTONE H-1 IN EUKARYOTE CHROMATIN - 3 STRUCTURAL REGIONS OF HISTONE H-1 MOLECULE [J].

HARTMAN, PG ;

CHAPMAN, GE ;

MOSS, T ;

BRADBURY, EM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 77 (01) :45-51

[7]

HENRICKSON WA, 1989, SYNCHROTRON RAD STRU, P317

[8] CALF THYMUS HISTONE H-1 IS A RECOMBINASE THAT CATALYZES ATP-INDEPENDENT DNA STRAND TRANSFER [J].

KAWASAKI, I ;

SUGANO, S ;

IKEDA, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5281-5285

[9] THE CHICKEN H5-GENE IS UNLINKED TO CORE AND H1-HISTONE GENES [J].

KRIEG, PA ;

ROBINS, AJ ;

DANDREA, R ;

WELLS, JRE .

NUCLEIC ACIDS RESEARCH, 1983, 11 (03) :619-627

[10] NUCLEOSOME STRUCTURE [J].

MCGHEE, JD ;

FELSENFELD, G .

ANNUAL REVIEW OF BIOCHEMISTRY, 1980, 49 :1115-1156

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