HIGH-LEVEL EXPRESSION OF FOREIGN GENES IN HANSENULA-POLYMORPHA

被引：29

作者：

GELLISSEN, G ^{[1
]}

JANOWICZ, ZA ^{[1
]}

WEYDEMANN, U ^{[1
]}

MELBER, K ^{[1
]}

STRASSER, AWM ^{[1
]}

HOLLENBERG, CP ^{[1
]}

机构：

[1] UNIV DUSSELDORF,INST MIKROBIOL,W-4000 DUSSELDORF 1,GERMANY

来源：

BIOTECHNOLOGY ADVANCES | 1992年 / 10卷 / 02期

关键词：

HETEROLOGOUS GENE EXPRESSION; SECRETION; GLYCOSYLATION; HANSENULA-POLYMORPHA;

D O I：

10.1016/0734-9750(92)90002-Q

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The methylotrophic yeast Hansenula polymorpha belongs to a limited number of non-Saccharomyces yeast species used as hosts for heterologous gene expression. It has successfully been applied for the production of hormones, antigens and enzymes. The system excells by mitotically stable recombinant strains, high productivity and faithful processing of the produced polypeptides. The favourable charcteristics of this microorganism for protein production at an industrial scale are described in the following article focusing on some recent representative examples.

引用

页码：179 / 189

页数：11

共 28 条

[1] YEAST SYSTEMS FOR THE COMMERCIAL PRODUCTION OF HETEROLOGOUS PROTEINS [J].

BUCKHOLZ, RG ;

GLEESON, MAG .

BIO-TECHNOLOGY, 1991, 9 (11) :1067-1072

[2] PICHIA-PASTORIS AS A HOST SYSTEM FOR TRANSFORMATIONS [J].

CREGG, JM ;

BARRINGER, KJ ;

HESSLER, AY ;

MADDEN, KR .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3376-3385

[3]

CREGG JM, 1987, BIOL RES IND YEASTS, V2, P1

[4] CLONING OF THE SCHWANNIOMYCES-OCCIDENTALIS GLUCOAMYLASE GENE (GAM1) AND ITS EXPRESSION IN SACCHAROMYCES-CEREVISIAE [J].

DOHMEN, RJ ;

STRASSER, AWM ;

DAHLEMS, UM ;

HOLLENBERG, CP .

GENE, 1990, 95 (01) :111-121

[5]

ELLIS SB, MOL CELL BIOL, V5, P111

[6] PRODUCTION AND IMMUNOLOGICAL ANALYSIS OF RECOMBINANT HEPATITIS-B VACCINE [J].

EMINI, EA ;

ELLIS, RW ;

MILLER, WJ ;

MCALEER, WJ ;

SCOLNICK, EM ;

GERETY, RJ .

JOURNAL OF INFECTION, 1986, 13 :3-&

[7] HETEROLOGOUS GENE-EXPRESSION IN HANSENULA-POLYMORPHA - EFFICIENT SECRETION OF GLUCOAMYLASE [J].

GELLISSEN, G ;

JANOWICZ, ZA ;

MERCKELBACH, A ;

PIONTEK, M ;

KEUP, P ;

WEYDEMANN, U ;

HOLLENBERG, CP ;

STRASSER, AWM .

BIO-TECHNOLOGY, 1991, 9 (03) :291-295

[8]

GELLISSEN G, IN PRESS A VANLEEUWE

[9]

GLEESON MA, 1986, J GEN MICROBIOL, V132, P3459

[10] THE METHYLOTROPHIC YEASTS [J].

GLEESON, MA ;

SUDBERY, PE .

YEAST, 1988, 4 (01) :1-15

← 1 2 3 →