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AN EPITOPE WITHIN THE DNA-BINDING DOMAIN OF THE HERPES-SIMPLEX VIRUS IMMEDIATE-EARLY PROTEIN VMW175 IS CONSERVED IN THE VARICELLA-ZOSTER VIRUS GENE 62 PROTEIN
被引:15
作者:
EVERETT, R
CROSS, A
TYLER, J
ORR, A
机构:
[1] MRC Virology Unit, Glasgow G11 5JR, Church Street
关键词:
D O I:
10.1099/0022-1317-74-9-1955
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
We have isolated a panel of monoclonal antibodies that recognize the DNA-binding domain of the herpes simplex virus type 1 (HSV-1) immediate early polypeptide Vmw175. The mice used for the fusions had been immunized with the isolated Vmw175 DNA-binding domain. This had been purified from bacteria that carried a phage T7 expression plasmid with the DNA-binding domain coding region. The epitopes recognized by the monoclonal antibodies were mapped by using a family of truncated versions of the DNA-binding domain, which had also been expressed in the bacterial expression system. The monoclonal antibodies divided into at least four different groups according to this mapping. Several of the monoclonal antibodies recognized Vmw175 expressed in infected BHK cells by HSV-1 strain 17 in Western blots. One of them also recognized the corresponding protein of varicella-zoster virus gene 62. This is further illustration of the relatedness of the two polypeptides.
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页码:1955 / 1958
页数:4
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