SHAPE OF PROTEIN-S3 AND PROTEIN-S17 FROM THE SMALL SUBUNIT OF ESCHERICHIA-COLI RIBOSOME

被引：4

作者：

FRANZ, A ^{[1
]}

GEORGALIS, Y ^{[1
]}

GIRI, L ^{[1
]}

机构：

[1] MAX PLANCK INST MOLEC GENET,ABT WITTMAN BERLIN DAHLEM,D-1000 BERLIN 33,FED REP GER

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 578卷 / 02期

关键词：

(Escherichia coli); Hydrodynamic properties; Ribosome; S17; protein; S3;

D O I：

10.1016/0005-2795(79)90167-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The shapes of proteins S3 and S17 purified from the 30 S subunit of Escherichia coli A19 were studied by hydrodynamic methods. The proteins have s20,w0 values of 2.1 ± 0.1 S and 1.2 ± 0.1 S and D20,w0 values of 7.4 ± 0.5 · 10-7 cm2/s and 11.4 ± 0.6 · 10-7 cm2/s. The respective molecular weights determined by sedimentation equilibrium are 25 800 ± 500 and 9900 ± 300. The intrinsic viscosity values for the two proteins are 5.8 ± 0.3 ml/g and 4.2 ± 0.2 ml/g. From these hydrodynamic parameters a slightly elongated shape for S3 and a globular shape for S17 have been concluded. © 1979.

引用

页码：365 / 371

页数：7

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