INTERACTION OF INTERLEUKIN-5 WITH ITS RECEPTORS ON MURINE LEUKEMIC BCL1 CELLS AND ITS IMPLICATION IN BIOLOGICAL-ACTIVITY

被引：17

作者：

TSURUOKA, N

FUNAKOSHI, K

KODAMA, S

TSUJIMOTO, M

机构：

[1] Suntory Institute for Biomedical Research, Mishima, Osaka

来源：

CELLULAR IMMUNOLOGY | 1990年 / 125卷 / 02期

关键词：

D O I：

10.1016/0008-8749(90)90090-E

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Interaction of interleukin (IL)-5 with its receptors on murine leukemic cell line, BCL1 cells was examined. 125I-labeled recombinant murine IL-5(rmIL-5) bound specifically to high-affinity receptors on BCL1 cells. rmIL-5, which was about 2500-fold more active than recombinant human IL-5(rhIL-5) in IgM-inducing activity on BCL1 cells, also showed about 5000-fold higher affinity to receptors. These results suggest that the bioactivity of IL-5 correlates with its receptor-binding activity. When disulfide bond formation was blocked, rmIL-5 dissociated into a monomer and lost its biological activity. This monomeric form of rmIL-5 also lost its ability to bind to cells, suggesting that dimer formation is essential for the biological activity of IL-5. © 1990.

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页码：354 / 362

页数：9

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