CONSERVATION OF INFECTIVITY IN PURIFIED FIBRILLARY EXTRACTS OF SCRAPIE-INFECTED HAMSTER BRAIN AFTER SEQUENTIAL ENZYMATIC DIGESTION OR POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：48

作者：

BROWN, P

LIBERSKI, PP

WOLFF, A

GAJDUSEK, DC

机构：

[1] Central Nervous Sys. Lab., Natl. Inst. Neuro. Disorders, National Institutes of Health, Bethesda

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 18期

关键词：

enzymes; scrapie amyloid protein; scrapie-associated fibrils;

D O I：

10.1073/pnas.87.18.7240

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Infectious extracts of scrapie-infected hamster brain enriched for scrapie-associated fibrils and scrapie amyloid protein (PrP) were partially denatured and subjected to either polyacrylamide gel electrophoresis with subsequent isolation of the PrP band or sequential enzymatic digestion with deglycosidase, phospholipase, proteinase, and several different nucleases. Infectivity measurements of these various specimens revealed a convincing association between infectivity and scrapie amyloid protein, with or without its sugar chains and disulfide bonds, and did not support the hypothesis that nucleic acid is involved in replication.

引用

页码：7240 / 7244

页数：5

共 49 条

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