GENETIC-ENGINEERING OF SURFACE ATTACHMENT SITES YIELDS ORIENTED PROTEIN MONOLAYERS

被引：66

作者：

STAYTON, PS ^{[1
]}

OLINGER, JM ^{[1
]}

JIANG, M ^{[1
]}

BOHN, PW ^{[1
]}

SLIGAR, SG ^{[1
]}

机构：

[1] UNIV ILLINOIS,BECKMAN INST ADV SCI & TECHNOL,405 N MATHEWS,URBANA,IL 61801

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1992年 / 114卷 / 24期

关键词：

D O I：

10.1021/ja00050a008

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

There has been considerable interest in the use of self-assembly and Langmuir-Blodgett techniques to generate ordered macromolecular monolayers. We describe a general method of using genetic engineering to produce a unique chemical group on the surface of a protein at a predefined site which can be used to orient the macromolecule in a self-assembled film. Using the heme protein cytochrome b5, produced from a totally synthetic gene, allows direct determination of orientation through measurement of absorbance linear dichroism.

引用

页码：9298 / 9299

页数：2

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