A NEW WAY OF BIOSENSING USING FLUORESCENCE ENERGY-TRANSFER AND LANGMUIR-BLODGETT-FILMS

被引：4

作者：

SIEGMUND, HU

BECKER, A

机构：

[1] Bayer AG, Zentrale Forschung Uerdingen

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1993年 / 11卷 / 1-3期

关键词：

D O I：

10.1016/0925-4005(93)85244-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method of detecting the binding of ligand molecules (mannosides) to a biological receptor (Con A) by Forster energy transfer from Langmuir-Blodgett (LB) films is presented. The LB films consist of pre-polymerized amphiphilic polymethacrylates containing a coumarin fluorophore (donor) and reactive groups to which biological receptors can be attached. Studies determining the stability of the system are described. The specific binding of rhodamine (acceptor)-labelled ligands and their competitive replacement by unlabelled analytes is demonstrated. A simple dual-channel fluorescence detector optimized for this purpose is described as well.

引用

页码：103 / 108

页数：6

共 28 条

[1] SPECIFIC INTERACTIONS OF PROTEINS WITH FUNCTIONAL LIPID MONOLAYERS - WAYS OF SIMULATING BIOMEMBRANE PROCESSES
AHLERS, M
MULLER, W
REICHERT, A
RINGSDORF, H
VENZMER, J
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1990, 29 (11) : 1269 - 1285
[2] PROTEIN INTERACTIONS WITH ORDERED LIPID FILMS - SPECIFIC AND UNSPECIFIC BINDING
AHLERS, M
BLANKENBURG, R
HAAS, H
MOBIUS, D
MOHWALD, H
MULLER, W
RINGSDORF, H
SIEGMUND, HU
[J]. ADVANCED MATERIALS, 1991, 3 (01) : 39 - 46
[3] AHLERS M, 1990, ANGEW CHEM, V102, P1310
[4] ANDERSON FP, 1988, CLIN CHEM, V34, P1417
[5] DUDA G, 1988, MAKROMOL CHEM-RAPID, V9, P495
[6] EROKHIN VV, 1989, STUD BIOPHYS, V132, P97
[7] ULTRATHIN ORGANIC FILMS - MOLECULAR ARCHITECTURES FOR ADVANCED OPTICAL, ELECTRONIC AND BIO-RELATED SYSTEMS
FUCHS, H
OHST, H
PRASS, W
[J]. ADVANCED MATERIALS, 1991, 3 (01) : 10 - 18
[8] CHARACTERIZATION OF LANGMUIR-BLODGETT OVERLAYERS BY TIME-OF-FLIGHT SECONDARY ION MASS-SPECTROMETRY
HAGENHOFF, B
BENNINGHOVEN, A
SIEGMUND, HU
HOLTKAMP, D
[J]. THIN SOLID FILMS, 1992, 210 (1-2) : 601 - 605
[9] ANALYSIS OF LANGMUIR-BLODGETT OVERLAYERS BY TIME-OF-FLIGHT SECONDARY ION MASS-SPECTROMETRY (TOF-SIMS)
HAGENHOFF, B
DEIMEL, M
BENNINGHOVEN, A
SIEGMUND, HU
HOLTKAMP, D
[J]. JOURNAL OF PHYSICS D-APPLIED PHYSICS, 1992, 25 (05) : 818 - 832
[10] HANSEN WP, 1985, Patent No. 150905

← 1 2 3 →