INTESTINAL SYNTHESIS AND ABSORPTION OF VITAMIN-B12 IN CHANNEL CATFISH

A feeding experiment conducted in a controlled environment and using a vitamin B12-deficient, but otherwise nutritionally complete, purified diet revealed that intestinal microorganisms in channel catfish synthesized .apprx. 1.4 ng vitamin B12/g body wt per day. Removal of Co from the diet or supplementation with an antibiotic (succinylsulfathiazole) significantly reduced the rate of intestinal synthesis and liver stores of vitamin B12. Radiolabeled vitamin B12 in the blood, liver, kidneys and spleen of fish fed 60Co in the diet indicated that the intestinally synthesized vitamin was absorbed by the fish. The primary route of absorption was directly from the digestive tract into the blood because coprophagy was prevented in the rearing aquariums and the amount of vitamin B12 dissolved in the aquarium water was too low for gill absorption. Dietary supplementation of vitamin B12 was not necessary for normal growth and erythrocyte formation in channel catfish in a 24 wk feeding period. A longer period, however, may have caused a vitamin deficiency since liver-stored vitamin B12 decreased between the 2nd-24th wk.