CHARACTERIZATION OF A MAIZE-INFECTING POTYVIRUS FROM SPAIN

被引：15

作者：

ACHON, MA ^{[1
]}

MEDINA, V ^{[1
]}

SHANKS, M ^{[1
]}

MARKHAM, P ^{[1
]}

LOMONOSSOFF, GP ^{[1
]}

机构：

[1] JOHN INNES INST, JOHN INNES CTR, DEPT VIRUS RES, NORWICH NR4 7UH, NORFOLK, ENGLAND

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 1994年 / 100卷 / 02期

关键词：

MAIZE DWARF MOSAIC VIRUS; NUCLEOTIDE SEQUENCE; POTYVIRUS;

D O I：

10.1007/BF01876248

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In order to characterise and classify an unknown maize-infecting potyvirus isolated from fields in northeast Spain, the entire coat protein gene and the C-terminal two-thirds of the large nuclear inclusion protein (NIb) gene were cloned and sequenced. Protein sequencing enabled the cleavage site between the two proteins to be deduced and also revealed that on storage the viral coat protein undergoes a specific degradation in which the N-terminal 39 amino acids are removed. Comparison of the nucleotide sequence of the 3' non-coding region of the viral RNA and the predicted amino acid sequence of the coat protein with the equivalent regions of other members of the potyvirus group revealed that the Spanish virus is closely related to maize dwarf mosaic virus strain A.

引用

页码：157 / 165

页数：9

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