SEQUENCE-TAGGED SITES (STSS) AS STANDARD LANDMARKERS IN THE RICE GENOME

被引：69

作者：

INOUE, T ^{[1
]}

ZHONG, HS ^{[1
]}

MIYAO, A ^{[1
]}

ASHIKAWA, I ^{[1
]}

MONNA, L ^{[1
]}

FUKUOKA, S ^{[1
]}

MIYADERA, N ^{[1
]}

NAGAMURA, Y ^{[1
]}

KURATA, N ^{[1
]}

SASAKI, T ^{[1
]}

MINOBE, Y ^{[1
]}

机构：

[1] NATL INST AGROBIOL RESOURCES,INST SOC TECHNOINNOVAT AGR FORESTRY & FISHERIES,TSUKUBA,IBARAKI 305,JAPAN

来源：

THEORETICAL AND APPLIED GENETICS | 1994年 / 89卷 / 06期

关键词：

STS; RFLP; RICE; GENETIC MAP; CODING REGION;

D O I：

10.1007/BF00223712

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Generating sequence-tagged sites (STSs) is a prerequisite to convert a genetic map to a physical map. With the help of sequence information from these STSs one can also isolate specific genes. For these purposes, we have designed PCR primer sets, of 20 bases each, by reference to sequences of restriction fragment length polymorphism (RFLP) landmarkers consisting of rice genomic clones. These markers were evenly distributed over the 12 chromosomes and were shown to be single copy by Southern-blot analysis. With improved PCR protocols, 63 standard STS landmarkers in the rice genome were generated. Similarity searches of all partial sequences of RFLP landmarkers by the FASTA algorithm showed that 2 of the 63 RFLP landmarkers, G357 and G385, contained part of the ORFs of aspartate aminotransferase and protein kinase, respectively.

引用

页码：728 / 734

页数：7

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