MALTODEXTRIN ACCEPTOR REACTIONS OF STREPTOCOCCUS-MUTANS 6715 GLUCOSYLTRANSFERASES

被引:28
作者
FU, DT [1 ]
ROBYT, JF [1 ]
机构
[1] IOWA STATE UNIV SCI & TECHNOL,DEPT BIOCHEM & BIOPHYS,AMES,IA 50011
关键词
D O I
10.1016/0008-6215(91)84130-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The maltodextrin (maltose through maltoheptaose) acceptor reactions of two Streptococcus mutans 6715 glucosyltransferases (GTF-1 and GTF-S) were studied. The acceptor product structures were determined by comparing them with the known structures of the acceptor products of Leuconostoc mesenteroides B-512FM dextransucrase (EC 2.4.1.5) and L. mesenteroides B-1355 alternansucrase (EC 2.4.1.140). When reacted with maltose (G2), both GTF-I and GTF-S transferred a D-glucopyranose from sucrose to the nonreducing glucosyl residue to give panose (6(2)-alpha-D-glucopyranosyl maltose). Panose then served as an acceptor to give two further acceptor products, 6(2)-alpha-isomaltosyl maltose and 6(2)-alpha-nigerosyl maltose. 6(2)-alpha-Isomaltosyl maltose then went on to serve as an acceptor to give a series of homologous acceptor products with isomaltodextrin chains attached to C-6 of the nonreducing-end residue of maltose, while 6(2)-alpha-nigerosyl maltose did not further react. When reacted with other maltodextrins (G3-G7), both GTF-I and GTF-S transferred a D-glucopyranose to C-6 of either the nonreducing-end or the reducing-end residues of the maltodextrins, forming alpha(1 --> 6) linkages. When D-glucopyranose was transferred to the nonreducing-end residue by GTF-I or GTF-S, the first product was also an acceptor to give the second product, which then served as an acceptor to give the third product, etc., to give a homologous series of products. When D-glucopyranose was transferred to the reducing-end residue, the acceptor product that formed did not readily serve as an acceptor, or served only as a very poor acceptor, to give a small amount of the next homologue, as was the case for G7 with GTF-S. In addition, GTF-I also transferred D-glucopyranose to the reducing-end or to the nonreducing-end residue of maltotriose, forming alpha(1 --> 3) linkages, to give 3(3)-alpha-D-glucopyranosyl maltotriose and 3(1)-alpha-D-glucopyranosyl maltotriose. Neither of these acceptor products further served as acceptors to give a homologous series. Under equivalent conditions of equimolar amounts of acceptor and sucrose, maltose and maltotriose are much better acceptors with GTF-I than they are with GTF-S, which is better than L. mesenteroides B-512FM dextransucrase. The three enzymes display significantly different efficiencies for the different maltodextrin acceptor reactions, GTF-I and GTF-S having much higher efficiencies than L. mesenteroides B-512FM dextransucrase.
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页码:201 / 211
页数:11
相关论文
共 13 条
[1]   INHIBITION-REACTION AND ACCEPTOR-REACTION STUDIES OF STREPTOCOCCUS-MUTANS 6715 GLUCOSYLTRANSFERASES WITH 3-DEOXYSUCROSE, 3-DEOXY-3-FLUOROSUCROSE, AND ALPHA-D-ALLOPYRANOSYL BETA-D-FRUCTOFURANOSIDE [J].
BINDER, TP ;
ROBYT, JF .
CARBOHYDRATE RESEARCH, 1986, 154 :229-238
[2]   DISPROPORTIONATION REACTIONS CATALYZED BY LEUCONOSTOC AND STREPTOCOCCUS GLUCANSUCRASES [J].
BINDER, TP ;
COTE, GL ;
ROBYT, JF .
CARBOHYDRATE RESEARCH, 1983, 124 (02) :275-286
[3]   ACCEPTOR REACTIONS OF ALTERNANSUCRASE FROM LEUCONOSTOC-MESENTEROIDES NRRL B-1355 [J].
COTE, GL ;
ROBYT, JF .
CARBOHYDRATE RESEARCH, 1982, 111 (01) :127-142
[4]   ACCEPTOR REACTIONS OF MALTODEXTRINS WITH LEUCONOSTOC-MESENTEROIDES B-512FM DEXTRANSUCRASE [J].
FU, D ;
ROBYT, JF .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 283 (02) :379-387
[5]  
FUKUI K, 1982, INFECT IMMUN, V37, P1
[6]   BIOLOGY, IMMUNOLOGY, AND CARIOGENICITY OF STREPTOCOCCUS-MUTANS [J].
HAMADA, S ;
SLADE, HD .
MICROBIOLOGICAL REVIEWS, 1980, 44 (02) :331-384
[7]   CHARACTERIZATION OF EXTRACELLULAR, WATER-INSOLUBLE ALPHA-D-GLUCANS OF ORAL STREPTOCOCCI BY METHYLATION ANALYSIS, AND BY ENZYMIC-SYNTHESIS AND DEGRADATION [J].
HARE, MD ;
SVENSSON, S ;
WALKER, GJ .
CARBOHYDRATE RESEARCH, 1978, 66 (OCT) :245-264
[8]   MILLIGRAM TO GRAM SCALE PURIFICATION AND CHARACTERIZATION OF DEXTRANSUCRASE FROM LEUCONOSTOC-MESENTEROIDES NRRL B-512F [J].
MILLER, AW ;
EKLUND, SH ;
ROBYT, JF .
CARBOHYDRATE RESEARCH, 1986, 147 (01) :119-133
[9]  
Montville T J, 1978, Adv Appl Microbiol, V24, P55, DOI 10.1016/S0065-2164(08)70636-1
[10]   RELATIVE, QUANTITATIVE EFFECTS OF ACCEPTORS IN THE REACTION OF LEUCONOSTOC-MESENTEROIDES-B-512F DEXTRANSUCRASE [J].
ROBYT, JF ;
EKLUND, SH .
CARBOHYDRATE RESEARCH, 1983, 121 (SEP) :279-286