CONSTRUCTION OF A PROMOTER-PROBE SHUTTLE VECTOR FOR ESCHERICHIA-COLI AND BREVIBACTERIA

被引：12

作者：

BARAK, I ^{[1
]}

KOPTIDES, M ^{[1
]}

JUCOVIC, M ^{[1
]}

SISOVA, M ^{[1
]}

TIMKO, J ^{[1
]}

机构：

[1] SLOVAK ACAD SCI, INST MOLEC BIOL, DEPT RECOMBINANT DNA TECHNOL, CS-84251 BRATISLAVA, CZECHOSLOVAKIA

来源：

GENE | 1990年 / 95卷 / 01期

关键词：

coryneform; neomycin resistance; plasmids; Recombinant DNA;

D O I：

10.1016/0378-1119(90)90424-P

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We constructed a promoter-probe vector, pJUP05, for brevibacteria and Escherichia coli based on the promoterless neomycin-resistance (neoR) gene from Tn5. This gene confers resistance to the aminoglycosides, kanamycin and neomycin. The promoter of the neoR gene was deleted and replaced by a suitable multiple cloning site. There are translation stop codons in all three reading frames upstream from the neoR gene. The plasmid contains functional origins of DNA replication for both brevibacteria and E. coli, and permits selection for chloramphenicol- and/or ampicillin-resistance markers. © 1990.

引用

页码：133 / 135

页数：3

共 12 条

[1] NUCLEOTIDE-SEQUENCE AND EXACT LOCALIZATION OF THE NEOMYCIN PHOSPHOTRANSFERASE GENE FROM TRANSPOSON TN5 [J].