INDUCTION OF OVULATION AND OOCYTE MATURATION OF AMPHIBIAN (RANA-DYBOWSKII) OVARIAN FOLLICLES BY PROTEIN-KINASE-C ACTIVATION INVITRO

被引:17
作者
KWON, HB
CHANG, KJ
YOO, YR
LEE, CC
SCHUETZ, AW
机构
[1] SEOUL NATL UNIV, DEPT BIOL, SEOUL 151742, SOUTH KOREA
[2] JOHNS HOPKINS UNIV, SCH HYG & PUBL HLTH, DEPT POPULAT DYNAM, BALTIMORE, MD 21205 USA
关键词
D O I
10.1095/biolreprod47.2.169
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We previously reported that protein kinase C (PKC) activation induced meiotic maturation (germinal vesicle breakdown, GVBD) of Rana dybowskii follicular oocytes cultured in vitro without hormone treatment. The experiments reported here were carried out to establish whether ovarian follicles ovulated in response to PKC activation during culture. A phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), was used for PKC activation. TPA addition (10-mu-M) to cultured ovarian fragments induced ovulation and maturation of the oocytes similar to that seen following addition of frog pituitary homogenate (FPH, 0.05 pituitary/ml) or progesterone (0.5-mu-g/ml). Such changes were not observed when ovarian fragments were treated with inactive phorbol ester. The time course of TPA-induced ovulation was similar to that produced by FPH-stimulated ovulation. Both TPA- and FPH-stimulated ovulation and maturation were blocked by treatment with cycloheximide, forskolin (an adenylate cyclase stimulator), and 1-(5-isoquinolinylsulfonyl)-2-methyl-piperazine (H-7; a PKC inactivator). FPH treatment markedly increased progesterone levels in the medium during ovarian fragment culture whereas TPA treatment failed to elevate progesterone levels. Thus, TPA treatment mimics FPH and progesterone in inducing ovulation and meiotic maturation in cultured amphibian ovarian fragments. The data strongly suggest that PKC plays an important role in regulating ovulation as well as in modulating amphibian oocyte maturation during follicular differentiation.
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页码:169 / 176
页数:8
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