CHARACTERIZATION OF SELF-ASSOCIATION OF A SOYBEAN PROTEINASE INHIBITOR BY MEMBRANE OSMOMETRY

被引：30

作者：

HARRY, JB

STEINER, RF

机构：

[1] Laboratory of Physical Biochemistry, Naval Medical Research Institute, National Naval Medical Center, Bethesda

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 12期

关键词：

D O I：

10.1021/bi00840a057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The self-association of a soybean trypsin and chymotrypsin inhibitor has been characterized by membrane osmometry. The association is found to be of the monomer ⇌ dimer type with little or no higher forms present. The extent of association increases with increased ionic strength and with decreased monomer charge. The ionic strength effect and the pH effect (below pH 6) are both found to be consistent with the theoretical electrostatic free-energy changes. © 1969, American Chemical Society. All rights reserved.

引用

页码：5060 / &

共 16 条

[1]

ARMSTRONG JL, 1968, CHARACTERIZATION MAC

[2] POLYMERIZATION-DEPOLYMERIZATION OF TOBACCO MOSAIC VIRUS PROTEIN .6. OSMOTIC PRESSURE STUDIES OF EARLY STAGES OF POLYMERIZATION [J].

BANERJEE, K ;

LAUFFER, MA .

BIOCHEMISTRY, 1966, 5 (06) :1957-&

[3] A PURE TRYPSIN INHIBITOR FROM SOYA BEANS [J].

BIRK, Y ;

GERTLER, A ;

KHALEF, S .

BIOCHEMICAL JOURNAL, 1963, 87 (02) :281-&

[4] EXAMINATION OF DISSOCIATION OF MULTICHAIN PROTEINS IN GUANIDINE HYDROCHLORIDE BY MEMBRANE OSMOMETRY [J].

CASTELLINO, FJ ;

BARKER, R .

BIOCHEMISTRY, 1968, 7 (06) :2207-+

[5] SOYBEAN INHIBITORS .2. PREPARATIVE ELECTROPHORETIC PURIFICATION OF SOYBEAN PROTEINASE INHIBITORS ON POLYACRYLAMIDE GEL [J].

FRATTALI, V ;

STEINER, RF .

ANALYTICAL BIOCHEMISTRY, 1969, 27 (02) :285-&

[6]

FRATTALI V, 1969, J BIOL CHEM, V244, P274

[7]

HAYNES R, 1967, J BIOL CHEM, V242, P5378

[8]

HOCHSTRASSER K, 1969, Z PHYSIOL CHEM, V350, P655

[9] MONODISPERSITY AND QUATERNARY STRUCTURE OF GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE [J].

JAENICKE, R ;

SCHMID, D ;

KNOF, S .

BIOCHEMISTRY, 1968, 7 (03) :919-&

[10] PROPERTIES OF CHROMATOGRAPHICALLY PURIFIED TRYPSIN INHIBITORS FROM LIMA BEANS [J].

JONES, G ;

STEIN, WH ;

MOORE, S .

BIOCHEMISTRY, 1963, 2 (01) :66-&

← 1 2 →