2-STRUCTURALLY CLOSELY RELATED POLYPEPTIDES ENCODED BY 14-S MESSENGER-RNA ISOLATED FROM RAT LENS

被引：24

作者：

COHEN, LH ^{[1
]}

WESTERHUIS, LW ^{[1
]}

SMITS, DP ^{[1
]}

BLOEMENDAL, H ^{[1
]}

机构：

[1] KATHOLIEKE UNIV NIJMEGEN, DEPT BIOCHEM, BIOCHEM LAB, NL-6525 NIJMEGEN, NETHERLANDS

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1978年 / 89卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1978.tb20920.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

14‐S mRNA from rat lens codes for two subunits of α‐crystallin, A2 (Mr 20000) and AIns (Mr 24000, previously referred to as αX). Structural relationship between both translation products has been proved by immunoprecipitation with antisera directed against the different crystallin classes. Competition immunoprecipitation showed that the 14‐S mRNA translation products are precipitated by common antibodies, specific for the A subunit of α‐crystallin. Two‐dimensional gel electrophoresis and peptide analysis provided further evidence that the 24000‐Mr polypeptide, synthesized in vitro under direction of 14‐S mRNA, is identical with native αAIns. Although the structures of αA2 and αAIns are very similar, no precurosor‐product relationship exists between both 14‐S‐mRNA‐encoded polypeptides. Copyright © 1978, Wiley Blackwell. All rights reserved

引用

页码：251 / 258

页数：8

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