INTRACELLULAR FREE MAGNESIUM AND HIGH-ENERGY PHOSPHATES IN THE PERFUSED NORMOTENSIVE AND SPONTANEOUSLY HYPERTENSIVE RAT-HEART - A P-31 NMR-STUDY

被引：67

作者：

JELICKS, LA ^{[1
]}

GUPTA, RK ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PHYSIOL & BIOPHYS,1300 MORRIS PK AVE,BRONX,NY 10461

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1991年 / 4卷 / 02期

关键词：

SPONTANEOUSLY HYPERTENSIVE RATS; MAGNESIUM; BLOOD PRESSURE; PHOSPHORYLATION POTENTIAL; HIGH ENERGY PHOSPHATES;

D O I：

10.1093/ajh/4.2.131

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

We have employed P-31 nuclear magnetic resonance (NMR) spectroscopy to examine the relationship between cytosolic free Mg2+ ([Mg2+]in), intracellular pH, high energy phosphates, and genetic hypertension using the Wistar-Kyoto rat (WKY) as a control and the spontaneously hypertensive rat (SHR) as a model for essential hypertension. The mean systolic blood pressures (measured using the tail cuff method) of control and hypertensive rats (aged 7 to 12 weeks) were 113 +/- 4 mm Hg (mean +/- 2 SE, n = 14) and 162 +/- 5 mm Hg (mean +/- 2 SE, n = 17), respectively. Intracellular free Mg2+ levels were significantly depleted in the isolated Langendorff perfused hypertensive rat hearts (452 +/- 39-mu-mol/L, mean +/- 2 SE, n = 17) compared to control hearts (756 +/- 52-mu-mol/L, n = 14); however, intracellular pH did not differ in the SHR hearts (7.02 +/- 0.03, means +/- 2 SE, n = 7) compared with controls (7.03 +/- 0.03, n = 7). Although we could not demonstrate a statistically significant difference in the levels of P-creatine or ATP, intracellular P(i) was two-fold higher (5.71 +/- 2.28 mmol/L v 2.92 +/- 0.66 mmol/L, n = 4) and the phosphorylation potential, [MgATP]/[MgADP][P(i)], was correspondingly lower (3.0 x 10(4) +/- 1.6 x 10(4) v 8.3 x 10(4) +/- 1.4 x 10(4) (mol/L)-1, n = 4) in SHR compared to WKY hearts. These data demonstrate free magnesium depletion in heart muscle and indicate an alteration in cardiac bioenergetics in essential hypertension.

引用

页码：131 / 136

页数：6

共 34 条

[1]

Postnov Y.V., Orlov S.N., Ion transport across plasma membrane in primary hypertension, Physiol Rev, 65, pp. 904-945, (1985)

[2]

Bianchi G., Cusi D., Vezzoli G., Role of cellular sodium and calcium metabolism in the pathogenesis of essential hypertension, Semin Nephrol, 8, pp. 110-119, (1988)

[3]

Resnick L.M., Gupta R.K., Laragh J.H., Intracellular free magnesium in erythrocytes of essential hypertension: Relation to blood pressure and serum divalent cations, Proc Natl Acad Sci USA, 81, pp. 6511-6515, (1984)

[4]

Resnick L.M., Gupta R.K., Sosa R.E., Et al., Intracellular pH in human and experimental hypertension, Proc Natl Acad Sci USA, 84, pp. 7663-7667, (1987)

[5]

Matuura T., Kohno M., Kanayama K., Et al., Decreased intracellular free magnesium in erythrocytes of spontaneously hypertensive rats, Biochem Biophys Res Commun, 143, pp. 1012-1017, (1987)

[6]

Erne P., Bolli P., Burgisser E., Buhier F.R., Correlation of platelet calcium with blood pressure, N Engl J Med, 310, pp. 1084-1088, (1984)

[7]

Shibata S., Kuchii M., Taniguchi T., Calcium fluxes and binding in the aortic smooth muscle from the spontaneously hypertensive rat, Blood Vessels, 12, pp. 279-289, (1975)

[8]

Zsoter T.T., Wolchinsky C., Henein N.F., Ho L.C., Calcium kinetics in the aorta of spontaneously hypertensive rats, Cardiovasc Res, 11, pp. 353-357, (1977)

[9]

Jelicks L.A., Gupta R.K., NMR measurement of cytosolic free calcium, free magnesium, and intracellular sodium in the aorta of the normal and spontaneously hypertensive rat, J Biol Chem, 265, pp. 1394-1400, (1990)

[10]

Wexler L.F., Lorell B.H., Momura S., Et al., Enhanced sensitivity to hypoxia-induced diastolic dysfunction in pressure-overload left ventricular hypertrophy in the rat: Role of high energy phosphate depletion, Circ Res, 62, pp. 766-775, (1988)

← 1 2 3 4 →