A PLANT SIGNAL SEQUENCE ENHANCES THE SECRETION OF BACTERIAL CHIA IN TRANSGENIC TOBACCO

被引：31

作者：

LUND, P ^{[1
]}

DUNSMUIR, P ^{[1
]}

机构：

[1] DNA PLANT TECHNOL CORP, 6701 SAN PABLO AVE, OAKLAND, CA 94608 USA

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 18卷 / 01期

关键词：

CHITINASE; GLYCOSYLATION; PROTEIN SECRETION; TRANSGENIC PLANTS;

D O I：

10.1007/BF00018455

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When the secreted bacterial protein ChiA is expressed in transgenic tobacco, a fraction of the protein is glycosylated and secreted from the plant cells; however most of the protein remains inside the cells. We tested whether the efficiency of secretion could be improved by replacing the bacterial signal sequence with a plant signal sequence. We found the signal sequence and the first two amino acids of the PR1b protein attached to the ChiA mature protein directs complete glycosylation and secretion of the ChiA from plant cells. Glycosylation of this protein is not required for its efficient secretion from plant cells.

引用

页码：47 / 53

页数：7

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