CHARACTERIZATION OF A MONOCLONAL-ANTIBODY TO TURNIP MOSAIC-VIRUS AND ITS USE IN IMMUNODIAGNOSIS OF INFECTION

被引：5

作者：

HORSEWOOD, P ^{[1
]}

MCDERMOTT, MR ^{[1
]}

STOBBS, LW ^{[1
]}

BRAIS, PLJ ^{[1
]}

UNDERDOWN, BJ ^{[1
]}

机构：

[1] AGR CANADA, RES STN, VINELAND STN L0R 2E0, ONTARIO, CANADA

来源：

PHYTOPROTECTION | 1991年 / 72卷 / 02期

关键词：

D O I：

10.7202/706004ar

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Monoclonal antibodies specific for turnip mosaic virus (TuMV) were produced and used in a double antibody sandwich enzyme immunoassay to detect virus in infected plants. One particular antibody from a hybridoma clone having desirable growth, specificity and antibody production properties was characterized in detail. This antibody was shown by immunocytochemical electron microscopy and immunoblotting to react with a virion coat protein. Conditions providing efficient extraction of virus from leaves were investigated by using the antibody in both capture and detection steps of a sandwich immunoassay. With an extraction buffer system containing multiple detergents, a highly sensitive assay was produced that reliably detected virus in infected plants. This assay is now in routine use for immunodiagnosis of turnip mosaic virus infections.

引用

页码：61 / 68

页数：8

共 16 条

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