PURIFICATION AND CHARACTERIZATION OF A TRYPSIN-LIKE DIGESTIVE ENZYME FROM SPRUCE BUDWORM (CHORISTONEURA-FUMIFERANA) RESPONSIBLE FOR THE ACTIVATION OF DELTA-ENDOTOXIN FROM BACILLUS-THURINGIENSIS

被引：72

作者：

MILNE, R ^{[1
]}

KAPLAN, H ^{[1
]}

机构：

[1] UNIV OTTAWA, DEPT CHEM, OTTAWA K1N 6N5, ONTARIO, CANADA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1993年 / 23卷 / 06期

关键词：

SPRUCE BUDWORM; CHORISTONEURA-FUMIFERANA; SERINE PROTEASE; TRYPSIN; DELTA-ENDOTOXIN; BACILLUS-THURINGIENSIS;

D O I：

10.1016/0965-1748(93)90040-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A trypsin-like enzyme purified from spruce budworm (Choristoneura fumiferana) gut juice has a molecular mass of 25 kDa and its pH activity profile indicates a pK(a) of 8. Sequence homology with bovine trypsin of the N-terminus and active site, and the ionization dependence for catalysis, reflect the typical trypsin-like activities measured. The action of this enzyme (designated CFT-1) is compared to the neat gut juice with regard to the proteolytic activation of the delta-endotoxin from Bacillus thuringiensis.

引用

页码：663 / 673

页数：11

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