CARBOXYL-TERMINAL OF G-PROTEIN BETA-SUBUNIT IS REQUIRED FOR ASSOCIATION WITH GAMMA-SUBUNIT

被引：15

作者：

YAMAUCHI, J ^{[1
]}

KAZIRO, Y ^{[1
]}

ITOH, H ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL,MIDORI KU,YOKOHAMA,KANAGAWA 236,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 214卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2341

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate a complex formation of heterotrimeric G protein beta and gamma subunits, we constructed a deletion mutant which lacked the C-terminal WD40 repeating unit of beta(1) and cotransfected into HEK293 cells with gamma(2) which was tagged with FLAG-epitope. Co-immunoprecipitation assay indicated that the mutant beta(1) failed to associate with FLAG gamma(2). Furthermore, from the analysis of additional deletion mutants, the last 10 amino acids of C-terminal region of beta(1) were found to be necessary to associate with gamma(2). These results suggest that the C-terminal region, in addition to the N-terminal alpha-helical structure, of beta subunit may be involved in the beta gamma complex formation. (C) 1995 Academic Press, Inc.

引用

页码：694 / 700

页数：7

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