REDUCTION OF THYMINE BY LEUKOCYTES

被引：9

作者：

GOEDDE, HW

HOFFBAUER, R

BLUME, KG

机构：

[1] Institute of Human Genetics, University of Hamburg

[2] Department of Biochemical Genetics, Institute of Human Genetics and Anthropology, University of Freiburg/Br.

来源：

BIOCHEMICAL GENETICS | 1968年 / 2卷 / 01期

关键词：

D O I：

10.1007/BF01458454

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The maximal activity of the dihydrothymine dehydrogenase of fractions of human blood was found in leukocytes, especially in the supernatant after centrifugation of homogenates at 100,000 × g in the course of isolation of the cell components. The dihydrothymine dehydrogenase from human and pig leukocytes was purified tenfold with a yield of activity of about 60%. Gel filtration with Sephadex G-200 showed several peaks of enzymatic activity. A quantitative method for the estimation of thymine and dihydrothymine by means of thin layer chromatography and subsequent liquid scintillation counting is described. This method may have application in the study of the genetically determined β-aminoisobutyric acid excretion in man. © 1968 Plenum Publishing Corporation.

引用

页码：93 / +

页数：1

共 11 条

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