A NOVEL PEPTIDE FROM AMYLOID P COMPONENT SUPPORTS CELL ATTACHMENT

被引：27

作者：

DHAWAN, S ^{[1
]}

FIELDS, RL ^{[1
]}

ROBEY, FA ^{[1
]}

机构：

[1] NIDR,CELLULAR DEV & ONCOL LAB,PEPTIDE & IMMUNOCHEM UNIT,BETHESDA,MD 20892

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90825-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amyloid P component is a glycoprotein found in association with connective tissues throughout the body and is also a component of human serum. We have identified a dodecapeptide from amyloid P component which is capable of supporting the attachment of a wide variety of cells to the surface of polystyrene plastic dishes. 83% of the activity is confined to a hexapeptide, FTLCFR. Saturation of cell attachment occurs at a peptide concentration of 100 μg/ml used to coat the plastic. These results indicate that the active peptide may represent a functional property of amyloid P component which heretofore has no function. © 1990.

引用

页码：1284 / 1290

页数：7

共 8 条

[1] ULTRASTRUCTURE OF HUMAN AMYLOID AS REVEALED BY NEGATIVE STAINING TECHNIQUE [J].

BLADEN, HA ;

NYLEN, MU ;

GLENNER, GG .

JOURNAL OF ULTRASTRUCTURE RESEARCH, 1966, 14 (5-6) :449-&

[2]

DYCK RF, 1980, J EXP MED, V152, P1162, DOI 10.1084/jem.152.5.1162

[3] TISSUE SULFHYDRYL GROUPS [J].

ELLMAN, GL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77

[4] A PENTAPEPTIDE FROM THE LAMININ-B1 CHAIN MEDIATES CELL-ADHESION AND BINDS THE 67000-LAMININ RECEPTOR [J].

GRAF, J ;

OGLE, RC ;

ROBEY, FA ;

SASAKI, M ;

MARTIN, GR ;

YAMADA, Y ;

KLEINMAN, HK .

BIOCHEMISTRY, 1987, 26 (22) :6896-6900

[5] IDENTIFICATION OF AN AMINO-ACID-SEQUENCE IN LAMININ MEDIATING CELL ATTACHMENT, CHEMOTAXIS, AND RECEPTOR-BINDING [J].

GRAF, J ;

IWAMOTO, Y ;

SASAKI, M ;

MARTIN, GR ;

KLEINMAN, HK ;

ROBEY, FA ;

YAMADA, Y .

CELL, 1987, 48 (06) :989-996

[6] ISOLATION AND CHARACTERIZATION OF THE COMPLETE COMPLEMENTARY AND GENOMIC DNA-SEQUENCES OF HUMAN-SERUM AMYLOID-P-COMPONENT [J].

OHNISHI, S ;

MAEDA, S ;

SHIMADA, K ;

ARAO, T .

JOURNAL OF BIOCHEMISTRY, 1986, 100 (04) :849-858

[7] SIMPLIFICATION OF PROTEIN ASSAY METHOD OF LOWRY ET AL - WHICH IS MORE GENERALLY APPLICABLE [J].

PETERSON, GL .

ANALYTICAL BIOCHEMISTRY, 1977, 83 (02) :346-356

[8] AUTOMATED SYNTHESIS OF N-BROMOACETYL-MODIFIED PEPTIDES FOR THE PREPARATION OF SYNTHETIC PEPTIDE POLYMERS, PEPTIDE PROTEIN CONJUGATES, AND CYCLIC-PEPTIDES [J].

ROBEY, FA ;

FIELDS, RL .

ANALYTICAL BIOCHEMISTRY, 1989, 177 (02) :373-377

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