TRANSFORMATION OF VICIA-NARBONENSIS VIA AGROBACTERIUM-MEDIATED GENE-TRANSFER

被引：1

作者：

PICKARDT, T

MEIXNER, M

SCHADE, V

SCHIEDER, O

机构：

来源：

PLANT CELL REPORTS | 1991年 / 9卷 / 10期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Shoot tips and epicotyl-segments of Vicia narbonensis were co-cultivated with Agrobacterium tumefaciens strain C58C1 pGV 3850 HPT, carrying a plasmid coding for hygromycin-phosphotransferase. On callus-induction medium containing 60 mg/1 hygromycin for selection, approximately 18% of the explants produced hygromycin-resistant callus. After transfer to regeneration-medium these calluses produced hygromycin-resistant and nopalin-positive somatic embroyos which could be regenerated to plantlets. The integration of the T-DNA into the plant genome was confirmed by Southern analysis.

引用

页码：535 / 538

页数：4

共 18 条

[1] PLANT-REGENERATION FROM CALLUS-CULTURES OF SEVERAL SOYBEAN GENOTYPES VIA EMBRYOGENESIS AND ORGANOGENESIS
BARWALE, UB
KERNS, HR
WIDHOLM, JM
[J]. PLANTA, 1986, 167 (04) : 473 - 481
[2] AGROBACTERIUM-TUMEFACIENS-MEDIATED TRANSFORMATION OF PISUM-SATIVUM L USING BINARY AND COINTEGRATE VECTORS
DEKATHEN, A
JACOBSEN, HJ
[J]. PLANT CELL REPORTS, 1990, 9 (05) : 276 - 279
[3] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
FEINBERG, AP
VOGELSTEIN, B
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13
[4] Hildebrand EM, 1934, J AGRIC RES, V48, P0857
[5] PRODUCTION OF TRANSGENIC SOYBEAN PLANTS USING AGROBACTERIUM-MEDIATED DNA TRANSFER
HINCHEE, MAW
CONNORWARD, DV
NEWELL, CA
MCDONNELL, RE
SATO, SJ
GASSER, CS
FISCHHOFF, DA
RE, DB
FRALEY, RT
HORSCH, RB
[J]. BIO-TECHNOLOGY, 1988, 6 (08): : 915 - 921
[6] Kupila-Ahvenniemi S, 1968, Adv Morphog, V7, P45
[7] Lazzeri PA, 1985, PLANT MOL BIOL REP, V3, P160, DOI DOI 10.1007/BF02886752
[8] Maniatis T., 1982, MOL CLONING
[9] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES
MURASHIGE, T
SKOOG, F
[J]. PHYSIOLOGIA PLANTARUM, 1962, 15 (03) : 473 - 497
[10] PARROT WA, 1988, NEWSLETTER IAPTC, V54, P10

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