INTERMOLECULAR ELECTRON-TRANSFER FROM QUINOPROTEINS AND ITS RELEVANCE TO BIOSENSOR TECHNOLOGY

Long-range intermolecular electron transfer occurs between the redox centers of the quinoprotein methylamine dehydrogenase and the copper protein amicyanin. A steady-state kinetic assay was developed and used to describe factors that are relevant to this process. Kinetic, physical. and structural data on these proteins and the complexes which they form are providing a picture of how nature accomplishes long-range electron transfer through proteins, a process that is critical to the development of direct amperometric biosensors.